Enhanced Expression of Matrix Metalloproteinase-9 in Abdominal Aortic Aneurysms
Identifieur interne : 003570 ( Main/Exploration ); précédent : 003569; suivant : 003571Enhanced Expression of Matrix Metalloproteinase-9 in Abdominal Aortic Aneurysms
Auteurs : Akimasa Yamashita [Japon] ; Takafumi Noma [Japon] ; Atsushi Nakazawa [Japon] ; Satoshi Saito [Japon] ; Kentaro Fujioka [Japon] ; Nobuya Zempo [Japon] ; Kensuke Esato [Japon]Source :
- World Journal of Surgery [ 0364-2313 ] ; 2001-03-01.
Abstract
Abstract.: Abdominal aortic aneurysms (AAAs) are characterized by structural alterations of the aortic wall resulting from degradation of collagen and elastin. Matrix metalloproteinases (MMPs), particularly MMP-2 and MMP-9, show strong elastinolytic activity. We examined the levels of mRNA for MMP-2, MMP-9, membrane type (MT)-MMP-1, tissue inhibitor of metalloproteinase-1 (TIMP-1), and TIMP-2 in AAAs (n= 8), atherosclerotic occlusive diseases (AOD) (n= 8), and normal subjects (n= 8) using the reverse transcription-polymerase chain reaction (RT-PCR). We also analyzed the gelatinolytic activity of these metalloproteinases using gelatin zymography. The levels of MMP-2 and MMP-9 mRNA were increased in the AAA group compared with those in the AOD group and normal subjects. The levels for TIMP-1 and TIMP-2 mRNA in the AAA group were also higher than those in the AOD and normal groups. Only in the case of MT-MMP-1 was the difference between AAA and AOD not statistically significant. By gelatin zymography with the same samples used for RT-PCR, gelatinolytic activity of MMP-9 was elevated in all AAA tissues. The 62-kDa form of MMP-2 was elevated in both the AAA and AOD groups and did not differ significantly between them. Linear regression analysis demonstrated a significant positive correlation between mRNA levels of MMPs and those of TIMPs. These observations suggest that aneurysm formation in patients with atherosclerosis is related to the degree of MMP-9 expression.
Url:
DOI: 10.1007/s002680020062
Affiliations:
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Surg.</title><idno type="ISSN">0364-2313</idno><idno type="eISSN">1432-2323</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="2001-03-01">2001-03-01</date><biblScope unit="volume">25</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="259">259</biblScope><biblScope unit="page" to="265">265</biblScope></imprint><idno type="ISSN">0364-2313</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0364-2313</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract.: Abdominal aortic aneurysms (AAAs) are characterized by structural alterations of the aortic wall resulting from degradation of collagen and elastin. Matrix metalloproteinases (MMPs), particularly MMP-2 and MMP-9, show strong elastinolytic activity. We examined the levels of mRNA for MMP-2, MMP-9, membrane type (MT)-MMP-1, tissue inhibitor of metalloproteinase-1 (TIMP-1), and TIMP-2 in AAAs (n= 8), atherosclerotic occlusive diseases (AOD) (n= 8), and normal subjects (n= 8) using the reverse transcription-polymerase chain reaction (RT-PCR). We also analyzed the gelatinolytic activity of these metalloproteinases using gelatin zymography. The levels of MMP-2 and MMP-9 mRNA were increased in the AAA group compared with those in the AOD group and normal subjects. The levels for TIMP-1 and TIMP-2 mRNA in the AAA group were also higher than those in the AOD and normal groups. Only in the case of MT-MMP-1 was the difference between AAA and AOD not statistically significant. By gelatin zymography with the same samples used for RT-PCR, gelatinolytic activity of MMP-9 was elevated in all AAA tissues. The 62-kDa form of MMP-2 was elevated in both the AAA and AOD groups and did not differ significantly between them. Linear regression analysis demonstrated a significant positive correlation between mRNA levels of MMPs and those of TIMPs. These observations suggest that aneurysm formation in patients with atherosclerosis is related to the degree of MMP-9 expression.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Yamashita, Akimasa" sort="Yamashita, Akimasa" uniqKey="Yamashita A" first="Akimasa" last="Yamashita">Akimasa Yamashita</name></noRegion><name sortKey="Esato, Kensuke" sort="Esato, Kensuke" uniqKey="Esato K" first="Kensuke" last="Esato">Kensuke Esato</name><name sortKey="Fujioka, Kentaro" sort="Fujioka, Kentaro" uniqKey="Fujioka K" first="Kentaro" last="Fujioka">Kentaro Fujioka</name><name sortKey="Nakazawa, Atsushi" sort="Nakazawa, Atsushi" uniqKey="Nakazawa A" first="Atsushi" last="Nakazawa">Atsushi Nakazawa</name><name sortKey="Noma, Takafumi" sort="Noma, Takafumi" uniqKey="Noma T" first="Takafumi" last="Noma">Takafumi Noma</name><name sortKey="Saito, Satoshi" sort="Saito, Satoshi" uniqKey="Saito S" first="Satoshi" last="Saito">Satoshi Saito</name><name sortKey="Zempo, Nobuya" sort="Zempo, Nobuya" uniqKey="Zempo N" first="Nobuya" last="Zempo">Nobuya Zempo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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